Gel extraction manual

Catalog number: K The PureLink® Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments from TAE or TBE agarose gels of various percentages. DNA can be extracted and purified from agarose gels with different melting points in ∼30 minutes using PureLink® silica membrane-based quick gel extraction columns. PCR clean-up, gel extraction Reagents, consumables, and equipment to be supplied by user Reagents • 96– % ethanol Consumables • mL microcentrifuge tubes • Disposable pipette tips Equipment • Manual pipettors • Centrifuge for microcentrifuge tubes • Heating block, water bath, or thermomixer for gel extraction. 3. Determine the appropriate volume of the gel slice by weighing it in a clean mL microcentrifuge tube. Assuming a density of 1 g/mL, the volume of gel is derived as follows: a gel slice of mass g will have a volume of mL. 4. Add 1 volume Binding Buffer (XP2). 5. Incubate at 60°C for 7 minutes or until the gel has completely melted.

PCR clean-up, gel extraction Reagents, consumables, and equipment to be supplied by user Reagents • 96– % ethanol Consumables • mL microcentrifuge tubes • Disposable pipette tips Equipment • Manual pipettors • Centrifuge for microcentrifuge tubes • Heating block, water bath, or thermomixer for gel extraction. Agarose gel extraction only: Incubate mixture at 50°C with constant shaking or repeated orxing vte ervy e2–3 min until the gel is completely dissolved. + 2 vol NTI per 1 vol sample 2 Bind DNA Place a NucleoSpin® Gel and PCR Clean‑up XS Column into a Collection Tube (2 mL) and load up to μL sample. 3. Determine the appropriate volume of the gel slice by weighing it in a clean mL microcentrifuge tube. Assuming a density of 1 g/mL, the volume of gel is derived as follows: a gel slice of mass g will have a volume of mL. 4. Add 1 volume XP2 Binding Buffer. 5. Incubate at °C for 7 minutes or until the gel has completely melted.

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